Laboratoire de Génie Enzymatique et de Microbiologie LR03ES08 Ecole Nationale d Ingénieurs de Sfax Route Soukra, Km 3.5, B.P Sfax, TUNISIA

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1 Ecole Nationale d Ingénieurs de Sfax Laboratoire de recherche : Laboratoire de Génie Enzymatique et de Microbiologie LR03ES08 Ecole Nationale d Ingénieurs de Sfax Route Soukra, Km 3.5, B.P Sfax, TUNISIA Responsable : Pr. Moncef NASRI mon_nasri@yahoo.fr; moncef.nasri@enis.rnu.tn Tel.:

2 Présentation Générale du LGEM Le LGEM, créé en 2003, a pour mission de promouvoir une recherche pluridisciplinaire qui vise l acquisition de nouvelles connaissances scientifiques et de savoir-faire technologiques dans les domaines du Génie Biologique, principalement la Technologie Enzymatique et la Microbiologie Appliquée, disciplines scientifiques et techniques importantes des Biotechnologies. Le laboratoire a pour mission également de promouvoir la formation de scientifiques et d ingénieurs dans le domaine du Génie Biologique. Le laboratoire est dirigé par le Professeur Moncef NASRI et compte 30 personnes, dont une dizaine d enseignants-chercheurs qui encadrent des étudiants en thèse ou master. Le laboratoire regroupe plusieurs équipes de compétences complémentaires dans les domaines de la microbiologie, la biochimie, le Génie Enzymatique et le Génie Génétique. Le LGEM est un ensemble dans lequel les collaborations horizontales sont très importantes. Plusieurs chercheurs interviennent dans plusieurs thèmes. Thèmes de Recherche 1/ Les protéases microbiennes 2/ Les protéases digestives de poisson 3/ Les Biopeptides 4/: Valorisation biotechnologique de produits et co-produits agro-alimentaires et marins Projets : Coopération Tuniso-Marocaine ( ) : avec le Laboratoire de Chimie Agroalimentaire et Substances Naturelles Végétales et Marines (LCASVM), Département de Chimie, B.P Maarif N Casablanca Maroc. Mise en évidence de certaines activités biologiques dans les extraits et les hydrolysats protéiques d algues de Tunisie et du Maroc: Activités anti-oxydantes, anti-hypertensives et antimicrobiennes. CMCU : 08G908 avec le Laboratoire ECOLAG, Université Montpellier II, UMR 5119, CNRS France. Identification et caractérisation de protéases et de leurs propriétés (thermostabilité, résistance au sel) chez Arthrobacter arilaitensis à partir des données de la séquence génomique. ProBioGEM Lille France :

3 Hydrolysats peptidiques biologiquement actifs dérivés de protéines de co-produits de la pêche. Préparation et caractérisation, fractionnement et identification de peptides actifs : antioxidants, antihypertensifs, antimicrobiens, anti-thrombiques, etc. Publications Internationales (2003/2010) Bougatef Ali, Nedjar-Arroume Naima, Manni Laïla, Ravallec-Plé Rozenn, Barkia Ahmed, Guillochon Didier and Nasri Moncef (2010) Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinella aurita) by-products proteins. Food Chemistry, 118 : Bougatef Ali, Hajji Mohamed, Balti Rafik, Lassoued Imen, Triki-Ellouz Yosra and Nasri Moncef (2009) Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteases. Food Chemistry, 114 : Haddar Anissa, Bougatef Ali, Agrebi Rym, Sellami-Kamoun Alya and Nasri Moncef (2009) A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis A21. Purification and characterization. Process Biochemistry, 44 : Haddar Anissa, Agrebi Rym, Bougatef Ali, Hmidet Noomen, Sellami-Kamoun Alya and Nasri Moncef (2009) Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: Purification, characterization and potential application as a laundry detergent. Bioresource Technology 100 : Agrebi Rym, Haddar Anissa, Hmidet Noome, Jellouli Kemel, Manni Laïla and Nasri Moncef (2009) BSF1 fibrinolytic enzyme from a marine bacterium Bacillus subtilis A26: Purification, biochemical and molecular characterization. Process Biochemistry, 44 : Agrebi Rym, Haddar Anissa, Hajji Mohamed, Frikha Fakher, Manni Laila, Jellouli Kemel and Nasri Moncef (2009) Fibrinolytic enzymes from a newly isolated marine bacterium Bacillus subtilis A26 : Characterization and statistical media optimization. Canadian Journal of Microbiology, 55 : Fakhfakh Nahed, Kanoun Safia, Manni Laïla and Nasri Moncef (2009) Production, biochemical and molecular characterization of a keratinolytic serine-protease from a chicken feather-degrading Bacillus licheniformis RPk. Canadian Journal of Microbiology, 55 : Noomen Hmidet, Nedra El-Hadj Ali, Anissa Haddar, Safia Kanoun, Sellami-Kamoun Alya and Moncef Nasri (2009)

4 Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive. Biochemical Engineering Journal, 47 : Souissi Nabil, Bougatef Ali, Triki-Ellouz Yosra and Nasri Moncef (2009) Production of lipase and biomass by Staphylococcus simulans grown on sardinelle (Sardinella aurita) hydrolysates and peptone. African Journal of Biotechnology, 8 : Jellouli Kemel, Bougatef Ali, Daassi Dalel, Balti Rafik, Barkia Ahmed and Nasri Moncef (2009) New alkaline trypsin from the intestine of Grey triggerfish (Balistes capriscus) with high activity at low temperature: Purification and characterization. Food Chemistry, 116 : Jellouli Kemel, Bougatef Ali, Manni Laïla, Agrebi Rym, Rayda Siala, Younes Islem and Nasri Moncef (2009) Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1. Journal of Industrial Microbiology and Biotechnology, 36 : Balti Rafik, Barkia Ahmed, Bougatef Ali, Ktari Naourez and Nasri Moncef (2009) A heat-stable trypsin from the hepatopancreas of the cuttlefish (Sepia officinalis): Purification and characterization. Food Chemistry, 113 : Hajji Mohamed, Masmoudi Ons, Ellouz-Triki Yosra, Siala Rayda, Gharsallah Néji and Nasri Moncef (2009) Chemical composition and antioxidant and radical-scavenging activities of Periploca laevigata root bark extracts. Journal of the Science of Food and Agriculture, 89 : Syala Rayda, Sellami-Kamoun Alya, Hajji Mohamed, Abid Ines, Gharsallah Néji and Nasri Moncef (2009) Extracellular acid protease from Aspergillus niger I1: Purification and characterization. African Journal of Biotechnology, 8 : Bougatef Ali, Ravallec Rozenn, Nedjar-Arroume Naima, Barkia Ahmed, Guillochon Didier and Nasri Moncef (2009) Evidence of in vivo satietogen effect and control of food intake of smooth hound (Mustelus mustelus) muscle protein hydrolysate in rats. Journal of Functional Foods (In press). Barkia Ahmed, Bougatef Ali, Ben Khaled Hayet and Nasri Moncef (2009) Antioxidant activities of sardinelle heads and/or viscera protein hydrolysates prepared by enzymatic treatment. Journal of Food Biochemistry (In press). Manni Laila, Jellouli Kemel, Ghorbel-Bellaj Olfa, Agebi Rym, Haddar Anissa, Sellami- Kamoun Alya and Nasri Moncef (2009)

5 An oxidant- and solvent-stable protease produced by Bacillus cereus SV1: Application in the deproteinization of Shrimp wastes and as a laundry detergent additive. Applied Biochemistry and Biotechnology (In press). Fakhfakh-Zouari Nahed, Hmidet Noomen, Haddar Anissa, Kanoun Safia and Nasri Moncef (2009) A novel serine-metallo keratinase from a newly isolated Bacillus pumilus A1 grown on chicken feather meal: Biochemical and molecular characterization. Applied Biochemistry and Biotechnology (In press). Agrebi Rym, Hmidet Noomen, Hajji Mohamed, Haddar Anissa, Fakhfakh-Zouari Nahed and Nasri Moncef (2009) Fibrinolytic serine-protease from Bacillus amyloliquefaciens An6 grown on Mirabilis jalapa tuber powders: Production, partial purification and biochemical characterization. Applied Biochemistry and Biotechnolog (In press). El-Hadj Ali Nedra, Hmidet Noomen, Bougatef Ali, Nasri Rima and Nasri Moncef (2009) A laundry detergent-stable alkaline trypsin from striped seabream (Lithognathus mormyrus) viscera: Purification and characterization. Journal of Agricultural and Food Chemistry (In press). Bougatef Ali, Nedjar-Arroume Naima, Ravallec-Plé Rozenn, Leroy Yves, Guillochon Didier, Barkia Ahmed and Nasri Moncef (2008) Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita) by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases. Food Chemistry, 111 : Hmidet Noomen, Bayoudh Ahmed, Berrin J.G, Kanoun Safia, Juge Nathalie and Nasri Moncef (2008) Purification and biochemical characterization of a novel α-amylase from Bacillus licheniformis NH1. Cloning, nucleotide sequence and expression of amyn gene in Escherichia coli. Process Biochemistry, 43 : Manni Laila, Jellouli Kemel, Agrebi Rym, Bayoudh Ahmed and Nasri Moncef (2008) Biochemical and molecular characterization of a novel calcium-dependent metalloprotease from Bacillus cereus SV1. Process Biochemistry, 43 : Sellami-Kamoun Alya, Haddar Anissa, El-Hadj Ali Nedra, Ghorbel-Frikha Basma, Kanoun Safia and Nasri Moncef (2008) Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid laundry detergent formulations. Microbiological Rechearch, 163 : Souissi Nabil, Triki-Ellouz Yosra, Bougatef Ali, Blibech Monia and Nasri Moncef (2008)

6 Preparation and use of media for protease-producing bacterial strains based on by-products from Cuttlefish (Sepia officinalis) and wastewaters from marine-products processing factories. Microbiological Research, 163 : Hajji Mohamed, Rebai Ahmed, Gharsallah Néji and Nasri Moncef (2008) Optimization of alkaline protease production by Aspergillus clavatus ES1 in Mirabilis jalapa tuber-powder using statistical experimental design. Applied Microbiology and Biotechnology, 79 : Jellouli Kemel, Bayoudh Ahmed, Manni Laila, Agrebi Rym and Nasri Moncef (2008) Purification, biochemical and molecular characterisation of a metallo-protease from Pseudomonas aeruginosa MN7 grown on shrimp wastes. Applied Microbiology and Biotechnology, 79 : Bougatef Ali, Balti Rafik, Ben Zaied Saïda, Souissi Nabil and Nasri Moncef (2008) Pepsinogen and pepsin from the stomach of smooth hound (Mustellus mustellus): Purification, characterization and amino acid terminal sequences Food Chemistry, 107 : Ben Khaled Hayet, Bougatef Ali, Balti Rafik, Triki-Ellouz Yosra, Souissi Nabil and Nasri Moncef (2008) Isolation and characterization of trypsin from sardinelle (Sardinella aurita) viscecra. Journal of the Science of Food and Agriculture, 88 : Bougatef Ali, Souissi Nabil, Fakhfakh Nahad, Ellouz-Triki Yosra and Nasri Moncef (2007) Purification and characterization of trypsin from the viscera of Sardine (Sardina pilchardus) Food Chemistry, 102 : El-Hadj Ali Nedra, Agrebi Rym, Ghorbel-Frikha Basma, Sellami-Kamoun Alya, Kanoun Safia and Nasri Moncef (2007) Biochemical and molecular characterization of a detergent stable alkaline serine-protease from a newly isolated Bacillus licheniformis NH1. Enzyme and Microbial Technology, 40 : Hajji Mohamed, Kanoun Safia, Nasri Moncef and Gharsallah Néji (2007) Purification and characterization of an alkaline serine protease produced by a new isolated Aspergillus clavatus ES1. Process Biochemistry, 42 : Souissi Nabil, Bougatef Ali, Triki-Ellouz Yosra and Nasri Moncef (2007) Biochemical and functional properties of Sardinella (Sardinella aurita) by-product hydrolysates. Food Technology and Biotechnology, 45 : Ghorbel Sofiane, Souissi Nabil, Triki-Ellouz Yosra, Dufossé Laurent, Guérard Fabienne and Nasri Moncef (2005). Preparation and testing of Sardinella protein hydrolysates as nitrogen source for extracellular lipase production by Rhizopus oryzae World Journal of Microbiology and Biotechnology, 21 :

7 Ghorbel-Frikha Basma, Sellami-Kamoun Alya, Fakhfakh Nahed, Haddar Anissa, Manni Laila and Nasri Moncef. (2005). Production and purification of a calcium-dependent protease from Bacillus cereus BG1. Journal of Industrial Microbiology and Biotechnology. 32 : Ammar Emna, Nasri Moncef, Medhioub Khaled. (2005). Isolation of Enterobacteria able to degrade simple aromatic compounds from the wastewater from olive oil extraction. World Journal of Microbiology and Biotechnology, 21 : Chtourou Mohamed, Ammar Emna, Nasri Moncef and Medhioub K (2004). Isolation of a yeast, Trichosporon cutaneum, able to use low molecular weight phenolic compounds: application to olive mill waste water treatment. Journal of Chemical Technology and Biotechnology, 79 : Ghorbel Basma, Sellami-Kammoun Alya and Nasri Moncef (2003). Stability studies of protease from Bacillus cereus BG1. Enzyme and Microbial Technology, 32 : Khannous Lamia, Souissi Nabil, Ghorbel Basma, Jarboui Raja, Kallel Monem, Nasri Moncef and Gharsallah Néji (2003). Treatment of saline wastewaters from marine-products processing factories by activated sludge reactor. Environmental Technology, 24 : Triki-Ellouz Yosra, Ghorbel Basma, Souissi Nabil, Kammoun Sadok and Nasri Moncef (2003). Biosynthesis of protease by Pseudomonas aeruginosa MN7 grown on fish substrate. World Journal of Microbiology and Biotechnology, 19 : Hachicha Ridha, Rigane H, Ben Khodher M, Nasri Moncef and Medhioub Khaled (2003). Effects of partial stone removal on the co-composting of olive-oil processing solid residues with poultry manure and the quality of compost. Environmental Technology, 24 : Dufossé Laurent, Richert L, Nasri Moncef and Guérard Fafienne (2003). Microbial nitrogenous substrate from protein hydrolystae of non-tradable oysters, a marine equivalent to yeast extract. In «Marine Biotechnology : an overview of leading field», Colliec-Jouault S., Bergé J.P., Guézennec J., Fleurence J., Le Gal Y and Roy P. (eds), IFREMER, ISBN , Kherouatou Nawel, Nasri Moncef and Attia Hamadi (2003) A study of the Dromadery milk casein micelle and its changes during acidification. Brazilian Journal of Food Technology, 6 : Publications : Accepté avec des révisions mineures ou majeures Barkia Ahmed, Bougatef Ali, Nasri rima, Fetoui Emna, Balti Rafik, and Nasri Moncef (2009) Trypsin from the viscera of Bogue (Boops boops): Isolation and characterization.

8 Journal of the Science of Food and Agriculture. Haddar Anissa, Sellami-Kamoun Alya, Fakhfakh-Zouari Nahed, Hmidet Noomen, Fetoui Emna and Nasri Moncef (2009) Alkaline proteases from Bacillus mojavensis A21: Compatibility with laundry detergents and keratinolytic potential. Biochemical Engineering Journal. Fakhfakh-Zouari Nahed, Haddar Anissa, Frikha Fakher, Sellami-Kamoun Alya, Kanoun Safia and Nasri Moncef (2009) Degradation of poultry feathers and enzyme production by a newly isolated Bacillus pumilus A1. Process Biochemistry. Manni Laïla, Ghorbel-Bellaj Olfa, Jellouli Kemel and Nasri Moncef (2009). Isolation and characterization of chitin from shrimp wastes using enzymatic deproteinization by Bacillus cereus SV1 protease. Applied Biochemistry and Biotechnology. Ouvrage Recent Research Developments in Food By-products Technology and Biotechnology. Published by Research Signpost 2008/2009. Editor. Moncef NASRI Articles dans des ouvrages Ali Bougatef, Kemel Jellouli, Rafik Balti, Yosra Ellouz-Triki, Ahmed Barkia and Moncef Nasri Low molecular weight oxidant-stable protease from the intestine of smooth hound (Mustelus mustelus). Purification and characterization. In Progress in Food Chemistry (Editor: Ernst N. Koeffer). Nova Science Publishers Nedra El Hadj-Ali, Noomen Hmidet, Nabil Souissi, Alya Sellami-Kamoun and Moncef Nasri The use of fish powders and gruel as growth substrate for the production of alkaline serine proteases by Bacillus licheniformis NH1. In Food Sciences and Technology (Editors: Lorenzo V. Greco and Marco N. Bruno). Nova Science Publishers Eight Chapters in Recent Research Developments in Food By-products Technology and Biotechnology. Published by Research Signpost 2008/2009. Editor. Moncef NASRI. Publications Nationales : Plus que 20 publications. Communacations Nationales et Internationales : Plus que 200. Brevets : 9

9 La liste des membres du laboratoire de recherche Enseignants-chercheurs et personnels techniques Nom et prénom N C.I.N (1) Grade (2) Etablissement Temps consacré à la recherche NASRI Moncef Pr. ENIS 100 Diplôme en cours de préparation SELLAMI MC FSS 100 KAMOUN Alya ELLOUZ M.A ENIS 100 Habilitation TRIKI Yosra GHORBEL M.A FSGafsa 70 Habilitation Sofiane KRIAA Habib M.A FSS 10 Habilitation KAMMOUN M.A ESH-CM 100 Habilitation Sadok BARKIA M.A ESScTech Santé 100 Habilitation Ahmed GHORBEL MA ISBS 100 Habilitation FRIKHA Basma BAYOUDH MA FSS 100 Habilitation Ahmed BOUGATEF MA ISBS 100 Habilitation Ali El-HADJ ALI Dr 100 Habilitation Nedra MANNI Laila N ENIS 20 Habilitation HADDAR AsTechnologue ISET 100 Thèse Anissa JELLOULI AC ISBS 100 Thèse Kemel HAJJI Ayadi A FLSH Kairouan 30 Thèse OUERDA Hayet Pr principal détaché (Anglais) ENIS 30 - GHAZALA Technicienne ENIS 100 Dip Ingénieur

10 Imen Etudiants : Thèse Nom et prénom N CIN (5) Diplôme en préparation (6) Etablissement Nom et prénom de l encadreur Date de la première inscription AGREBI Rym Thèse ENIS NASRI Moncef Octobre 2004 SIALA - ELLEUCH Raïda Thèse FSS SELLAMI Alya VALLAEYS Tatiana Thèse ENIS NASRI Moncef KANOUN Safia Octobre 2004 FAKHFAKH- Octobre 2005 ZOUARI Nahed HMIDET Noomen Thèse ENIS NASRI Moncef Octobre 2005 KANOUN Safia HAJJI Mohamed Thèse FSS NASRI Moncef Octobre 2005 BALTI Rafik Thèse ENIS NASRI Moncef Octobre 2007 ARROUME Naïma BEN KHALED Thèse FSS NASRI Moncef Janvier 2008 Hayet GHORBEL Olfa Thèse ENIS NASRI Moncef Mars 2007 FETOUI Emna Thèse FSS BARKIA Ahmed NASRI Moncef Mars 2008 KTARI Nawrez Thèse ENIS NASRI Moncef Mai 2009 Etudiants : Mastère Nom et prénom N CIN (5) Diplôme en préparation (6) Etablissement Nom et prénom de l encadreur NASRI Rim Mastère ENIS BOUGATEF Ali MASMOUDI Ons Mastère ENIS NASRI Moncef HAJJI Mohamed Date de la première inscription MAALEJ Hana Mastère ENIS NASRI Moncef HMIDET Noomen LASSOUED Imen Mastère ENIS NASRI Moncef

11 ISLAM Younes Mastère/PFE ENIS NASRI Moncef DJERIDI Mourad Mastère ENIS BARKIA Ahmed SILA Assaad Mastère ENIS BOUGATEF Ali SELLIMI Sabrine Mastère ENIS HAJJI Mohamed CHTOUROU Ihsène Mastère INSAT SELLEMI Manel Mastère FSS NASRI Moncef HAMDI Mokhtar SELLAMI Kamoun Alya